Fig. 4

Cytokine expression by peripheral blood neutrophils and T cells is of limited polyfunctionality. The phenotypes of cells incubated with gamma-irradiated (dead) M. tuberculosis (Mtb) or viable Mtb-stimulated cells (Fig. 2) were assessed by Boolean gating. a Polyfunctional profiles in T cells and b neutrophils after stimulation with dead and viable M. tuberculosis. Uninfected animals (uninf; blue circles), 2-week postinfection animals (red circles), and 8-week postinfection infected (red triangles) were examined. c Mean proportion of T cell and neutrophil polyfunctional profiles per subset. d Cytokine expression profiles grouped into 1+, 2+, 3+, or 4+ cytokine phenotypes