Fig. 8

Spatial localization of T cell and neutrophil cytokine expression in lung granulomas. a Immunohistochemistry was performed on a necrotic granuloma, and the location of cytokine-positive T cells (blue) and neutrophils (red) was identified and plotted. The lymphocyte cuff, epithelioid macrophage region, and caseous center are included for context. Scale bars = 200 μm. b Boxes in the plotted positions (a) are included to show the staining characteristics for cytokine-positive T cells (blue) and neutrophils (green), arrowheads indicate cytokine + neutrophils and arrows indicate cytokine + T cells. Cytokine expression within each panel is indicated in red. Scale bar, lower right = 50 μm