Fig. 3
From: TOLLIP deficiency is associated with increased resistance to Legionella pneumophila pneumonia
WT and Tollip−/− macrophage cytokine responses after TLR and NLR stimulation. Bone marrow-derived macrophages (BMDM) were isolated from WT and Tollip−/− mice and stimulated with media, PAM3 (250 ng/ml), a TLR2/1 agonist, or LPS (10 ng/ml), TLR4 agonist, for 24 hr, and concentrations of a TNF and b IL-10 were measured from cellular supernatants. c We stimulated mouse BMDM with LPS 100 pg/ml for 2 h, then stimulated overnight with c media control or the NLRP3 ligand nigericin (100 µg/ml), and d NLRC4 ligand Salmonella flagellin (FliC, 100 ng/ml) or bovine serum albumin (100 ng/ml), coincubated with Lipofectamine 2000 to permit cytosolic translocation of each protein. IL-1β concentrations from cellular supernatants were measured by ELISA. Error bars are means ± SEM; statistical analyses were performed by paired t tests using Prism 7.0e. *p < 0.05, Student’s two-sided t test. The experiment shown is representative of three independent experiments, each conducted in triplicate
