Fig. 2 | Mucosal Immunology

Fig. 2

From: Small heat shock protein CRYAB inhibits intestinal mucosal inflammatory responses and protects barrier integrity through suppressing IKKβ activity

Fig. 2

Enforced expression of CRYAB inhibits LPS-induced production of inflammatory cytokines by THP-1 cells. a Expression of CRYAB in THP-1 cells transfected with CRYAB-expressing lentivirus was detected by qRT-PCR and immunoblotting. b, c Cytokine production in THP-1-pLVX and THP-1-CRYAB cells after LPS (100 ng/ml) stimulation for indicated time. RNA was isolated to assess the mRNA levels of proinflammatory cytokines (e.g., TNF-α, IL-6, IL-1β, and IL-8) by qRT-PCR. b Cytokines in the supernatants were measured by EILSA. *p < 0.05, **p < 0.01, ***p < 0.001. d Immunoblotting was performed to examine the levels of p-p65, p65, p-IκBα, and IκBα in THP-1-pLVX and THP-1-CRYAB cells after LPS stimulation for the indicated times. All data are shown from three independent experiments

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