Fig. 3

Enforced expression of CRYAB inhibits TNF-α-induced inflammatory response in HT29 and Caco-2 cells. a TNF-α decreased CRYAB in HT29 and Caco-2 cells. The cells were treated with TNF-α (10 ng/ml) for indicated times. CRYAB expression was assessed by qRT-PCR and immunoblotting; p < 0.05, **p < 0.01, ***p < 0.001. b HT29 and Caco-2 cells were transfected by CRYAB-expressing lentivirus. CRYAB level was assessed by qRT-PCR and immunoblotting; ***p < 0.001. c–f Expression of TNF-α, IL-6, IL-1β, and IL-8 was examined in CRYAB-overexpressing and control cells after exposed to TNF-α (10 ng/ml) for indicated times; *p < 0.05, **p < 0.01, ***p < 0.001. g Cytokines in the supernatants were measured by EILSA. **p < 0.01, ***p < 0.001. All data are shown from three independent experiments