Fig. 4

CRYAB inhibits the activation of the NF-κB pathway in HT29 and Caco-2 cells. a TNF-α-induced alterations in p-p65, p65, p-IκBα, and IκBα were examined by immunoblotting in the CRYAB-overexpressing and control cells. b NF-κB-driven luciferase was inhibited by CRYAB. CRYAB-overexpressing and control cells were transfected with a NF-κB-driven luciferase reporter. Dual-luciferase assays were performed after TNF-α stimulation (10 ng/ml) for indicated times. The firefly luciferase activity was measured with relative normalization for Renilla; *p < 0.05, **p < 0.01, ***p < 0.001. c Immunofluorescence staining of p65 in CRYAB-overexpressing and control cells after TNF-α treatment (10 ng/ml) for indicated times. Expression of CRYAB inhibited p65 nuclear localization. All data are shown from three independent experiments