Fig. 6
Antibiotic treatment abolishes Cybanmf333 susceptibility to colitis. Age-matched male and female WT and Cybanmf333 mice were treated with streptomycin from day −2 to day 6 (a). Body weight change (b), disease scores (c), survival (d), colon length (e), and histology scores (f) of treated WT and Cybanmf333 mice compared with untreated mice, all receiving DSS. 16S rRNA gene sequencing of fecal pellets collected at day −2 and day 0 from age-matched male and female mice. Inverse Simpson Diversity index (g), principal component analysis (h), and Bray–Curtis dendrogram showing sample clustering by similarity, with proportional abundance at the family level between mouse samples across rows (i). Proportional abundance of proteobacteria expressed as % of total sequences before and after treatment (j). Data are presented as mean ± SEM of three (a–f) or two (g–j) independent experiments. Each symbol represents an individual mouse; n = 19–27 for streptomycin/DSS groups and n = 5 for DSS only groups (b–f); n = 8–9 mice (g–j). Data were analyzed using two-way ANOVA followed by Bonferroni post hoc test (b, c), Mantel–Cox test (d), one-way ANOVA followed by Bonferroni multiple comparison test (e, f, j), or Mann–Whitney t-test (g). *P < 0.05, **P < 0.01, and ***P < 0.001 vs WT or relative control (b, c, e, f, j); °°P < 0.01 and °°°P < 0.001 vs WT, #P < 0.05 and ###P < 0.001 vs WT + Abx, ×P < 0.05, ××P < 0.01, and ×××P < 0.001 vs Cybanmf333 (b, c)
