Fig. 3 | Mucosal Immunology

Fig. 3

From: Role of RANK-L as a potential inducer of ILC2-mediated type 2 inflammation in chronic rhinosinusitis with nasal polyps

Fig. 3

The main source of RANK-L was immune cells in NPs. The presence of RANK-L-positive cells was assessed by immunohistochemistry. a Negative control antibody staining in NP is shown. b, c Representative immunostaining for RANK-L in NP (b) and control UT (c) are shown. d High-magnification image of (b) is shown. e The number of RANK-L-positive cells in control UT (n = 8) and NPs (n = 11) was counted. f (left) Representative-flow cytometric plots for RANK-L + cells in NPs are shown. We gated on single, live, RANK-L-positive (RANK-L + ) cells compared with isotype control IgG2b. f (right) RANK-L + cells were further separated into CD45 + and CD45− populations, and the frequency of RANK-L + cells in the two populations was calculated (n = 12). *p < 0.05 and ****p < 0.0001 were calculated by Mann–Whitney test (e) and pair t test (f)

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