Fig. 5

miR-219a-5p targets ETV5 in IBD. a Schematic representation of conserved duplexes formed by the interaction of ETV5 3′-UTR and miR-219a-5p. The phylogenetically conserved miR-219a-5p binding site is marked in red. b The relative luciferase activity of a reporter containing a wild type (WT) or mutant (Mut) ETV5 3′-UTR in HEK-293 T cells was detected after cotransfected with miR-219a-5p mimics or control mimics for 24 h. c Total RNA was extracted from CD4⁺ T cells as described in Fig. 3, and ETV5 expression was detected by qRT-PCR. *p < 0.05, **p < 0.01 versus LV-control transduced CD4⁺ T cells. d Colon biopsies were obtained from healthy controls (HC), CD and UC patients as mentioned in Fig. 1a, ETV5 expression was determined by qRT-PCR. **p < 0.01. e Representative colonic sections for ETV5 immunohistochemistry staining were obtained from healthy controls (HC, n = 10), patients with CD (CD, n = 8) and patients with UC (UC, n = 10). Data are representative of three independent experiments