Fig. 2
From: Lypd8 inhibits attachment of pathogenic bacteria to colonic epithelia
Immune responses to C. rodentium infection in Lypd8−/− mice. WT and Lypd8−/− mice were orally infected with 109 CFU of C. rodentium and analyzed on day 12 p.i. All results are pooled data from two separate experiments. a Quantitative reverse-transcription polymerase chain reaction (qRT-PCR)-based analysis of the expression of ROS-associated genes in colonic epithelial cells from uninfected and C. rodentium-infected WT and Lypd8−/− mice (n = 4 per uninfected group and 10 per infected group). Values were normalized to the expression of Gapdh. b Absolute numbers of CD4+ T cells, Th1 (IFNγ+) cells, Th17 (IL-17+) cells, and INFγ-producing Th17 (IFNγ+ IL-17+) cells in the colon (n = 7 per uninfected group and 6 per infected group). c qRT-PCR assay showing the expression of Cxcl1 and Cxcl2 relative to Gapdh in colonic epithelial cells from C. rodentium-infected and uninfected WT and Lypd8−/− mice (n = 7 per uninfected group and 10 per infected group). d Flow cytometry analysis of the abundance of neutrophils in the colon of uninfected and C. rodentium-infected WT and Lypd8−/− mice (n = 6–8 per group). Data represent the mean ± SD. A two-tailed Student’s t-test was performed for comparison of results between WT and Lypd8−/− mice. *p < 0.05, ***p < 0.005, ****p < 0.001, n.s: not significant
