Fig. 6
From: Lypd8 inhibits attachment of pathogenic bacteria to colonic epithelia
Lypd8 associates with intimin. a Schematic showing the domain structures of the C. rodentium and EHEC intimin proteins. b, c Enzyme-linked immunosorbent assays (ELISA) were performed to analyze the binding of FLAG-tagged Lypd8 and GST-tagged intimin. Increases in the optical density (OD), indicating mouse Lypd8 binding to each of the C. rodentium intimin domains, are shown by a line graph (b). The binding of human LYPD8 to EHEC intimin was also confirmed by increases in the OD (c). d Sensorgram of Biacore system investigating the binding between mouse Lypd8 and C. rodentium intimin and calculated kinetic parameters were shown. e ELISA analysis of the binding between unglycosylated mouse Lypd8 and individual domains of the intimin proteins from C. rodentium. b, c, and e Data represent the mean ± SD of triplicate samples. Representative results from three independent experiments are shown
