Fig. 7
From: Lypd8 inhibits attachment of pathogenic bacteria to colonic epithelia
Lypd8 inhibits the interaction between intimin and Tir from C. rodentium. a, b The effect of Lypd8 on the intimin-Tir interaction was assessed by ELISA. Changes in the OD, indicative of the binding of intimin to Tir in the presence of increasing concentrations of Lypd8, are shown in the line graph (a) and the bar graph (b). c–e The inhibitory function of Lypd8 on the interactions between the C-terminal domains of intimin (C-type lection domain (CTL) and Ig-like domain (Ig)) and Tir was analyzed by ELISA. The decrease in OD following the addition of increasing concentration of Lypd8 is shown in the line graph (c) and the bar graphs (d and e). Data represent the mean ± SD of triplicate samples. Representative results from three independent experiments are shown. f Sensorgrams of Biacore system investigating the binding of C. rodentium intimin and Tir in the presence of mouse Lypd8 protein and calculated kinetic parameters were shown. A two-tailed Student’s t-test was performed for comparison between groups. *p < 0.05, ***p < 0.005, ****p < 0.001
