Fig. 4

C-myc regulates the induction of T-bet in unconventional T cells. a Flow cytometric analysis of freshly isolated lymphocytes isolated from the epithelium of the small intestine from 4- to 5-month-old C-myc^Δ/ΔId2 mice and as controls C-myc^+/+ littermate mice after 3 weeks of tamoxifen treatment. Dot plot is pre-gated on DAPI⁻ CD45⁺ CD4⁻ and analyzed for TCRβ⁺ or TCRγδ⁺—as indicated—and bar diagram shows the percentages (Mean ± SEM) of at least eight mice per genotype. b Further analysis of co-expression of YFP and T-bet in IEL subsets. Bar diagram shows the percentages of YFP⁺ cells (Mean ± SEM). c Histograms represent T-bet expression of TCRβ⁺ and TCRγδ⁺ IELs and bar diagram shows MFI of T-bet expression (Mean ± SEM). d Flow cytometric analysis of hepatic lymphocytes of 3–5-month-old C-myc^Δ/ΔId2 mice and C-myc^+/+ littermate controls after 3 weeks of tamoxifen treatment. Bar diagram (right) shows the percentages of hepatic NKT cells (Mean ± SEM) of at least four mice per genotype. e Further flow cytometric analysis of hepatic NKT cells (TCRβ⁺ NK1.1⁺) for YFP and T-bet expression. Bar diagrams (right) show the percentages of YFP⁺ cells (Mean ± SEM). f Overlay histogram (left) shows representative T-bet expression of hepatic NKT cells (gated as in d, e) and bar diagram shows MFI of T-bet expression (Mean ± SEM)