Fig. 5

Unique regulon activities dictate colon MP bifurcation. a tSNE based on regulon activity scores of avg20. Colors are matched with the mRNA expression pattern-based tSNE plot in Fig. 1a. b Heatmap of regulon activity scores. Hierarchically clustered regulons are listed on right side (Red groups: high activity in macrophages; blue groups: high activity in dendritic cells). c Three regulon modules (M1–M3) are identified based on regulon connection specificity index (CSI). Close-up view of module M3 identifies sub-modules (sm1 - sm3) and their transcription factor regulons. Sankey plot represents the connectivity between d clusters (0–12) of colon phagocytes and regulon modules (M1-M3) or e macrophage clusters (1–4, 6, 7, and 11) and regulon sub-modules (sm1–sm3). f Plots of RAS and RSSZ (regulon specificity score Z-score) of the regulons in each cluster. Regulons with RAS > 0.2 & RSSZ > 1.0 were considered to be significant and labeled.