Table 1 Summary of colon MP populations.
From: Commensal microbiota drive the functional diversification of colon macrophages
Cluster | Top5 Cluster Gene Markers | Surface markers evaluated | mRNA for known MP genesa | Differentiation Status predicted from monocle2 | Specific active regulonsb | Referencesc |
---|---|---|---|---|---|---|
1 | Thbs1, Ccr2, Tgm2, Emilin2, Ifi27l2a | CD11c−CD206−CCR2+ | Ccr2 | Precursor cells (unaffected in GF mice) | Mxi1 | |
2 | Cx3cr1, Dst, H2-M2, Dnmt3a, Vcam1 | n.d.d | Itgax | Transional and mature cells (increased in GF mice) | Nfic, Tcf4, Mef2a, Ddit3, Nle2l2, Mitf, Fosb | |
3 | Apoe, Ms4a7, Cd63, Vcam1, Hpgds | n.d. | Itgax | Transitional cells (reduced in GF mice) | Spic, Nfic, Tcf4, Mef2a, Ddit3,Mitf, Fosb | |
4 | Pgf, Mmp13, Dnase1l3, Acp5, H2-M2 | CD11c+CD206intCCR2−CD121b+ | Itgax, Cd74, IL1r2, Cd9, Cd81 | Mature cells (reduced in GF mice) | Spic, Nifc,Tcf4, Pdrm1,Crem1,Fosb, Mxi1 | |
6 | Ccl7, Ccl2, F13a1, Pf4, Cd163 | CD11c−CD206hiCCR2−CD169+ | Mrc1(hi), Cd36, CD163,F13a1 | Mature cells (reduced in GF mice) | Jund, Tcd4, Nfic, Mafk, Atf4, Erg2, Mef2a | |
7 | Hspa1a, Hes1, Hspa1b, Ccl4, Jun | n.d. | Itgax | Mature cells (unaffected in GF mice) | Erg1, Erg2, Jun, Irf1, Atf3, Fosb, Mef2a, Nfic | |
11 | Ifit2, Ifit1, Rsad2, Isg15, Cxcl9 | n.d. | n.d. | Stat1, Stat2, Erg2, Irf1, Irf7 |