Fig. 4: Impact of SLC26A3 on cellular homeostasis.

a, b Representative western blot and densitometry analysis of SLC26A3 expression in CRISPR-mediated lentiviral transduced cells or Orf-mediated lentiviral transduced cells (n = 3). c Confocal images of SLC26A3 expression in vector controls, OE, and KD T84 IECs. Main image is a condensed z-stack and inserts depict the orthogonal view of the stacked image. d, e Analysis of extracellular pH following PMN transmigration over the course of 400 min in KD and OE T84 IECs (n = 6–8). f Analysis of extracellular pH following PMN transmigration over the course of 400 min in T84 IECs pretreated with 100 μM Ado for 24 h. g Change in pH between PMN exposed and non-PMN exposed IECs Vector, KD, OE, and 24 h Ado pretreated cells at T400. h Change in intracellular pH between T0 and T120 min in vector control, KD, and OE cells treated with 0, 10, or 100 μM Ado (n = 3). n = number of independent experiments performed, separate passages of cells were used for each experiment. The data for each experiment was pooled and expressed as the mean ± SEM and p-value determined by ANOVA or T-test, *p < 0.01, **p < 0.001.