Table 1 Strains used in this study.

SL1344

SB300

wildtype

Referred to as S. Tm

Sm

¹⁰⁶

SB300^TAG1

M3147

Tag1-aphT

Collectively referred to as WITS

Sm, Km

⁵⁹

SB300^TAG2

M3148

Tag2-aphT

Sm, Km

SB300^TAG11

M3149

Tag11-aphT

Sm, Km

SB300^TAG13

M3150

Tag13-aphT

Sm, Km

SB300^TAG17

M3151

Tag17-aphT

Sm, Km

SB300^TAG19

M3152

Tag19-aphT

Sm, Km

SB300^TAG21

M3153

Tag21-aphT

Sm, Km

S. Tm^Δ4

M566

ΔsipAsopBsopEsopE2

IEC invasion-deficient mutant

Sm

⁷¹

S. Tm^{Δ4 TAG1}

Z2557

ΔsipAsopBsopEsopE2, Tag1-aphT

Collectively referred to as WITS^Δ4

Sm, Km

This study

S. Tm^{Δ4 TAG2}

Z2558

ΔsipAsopBsopEsopE2, Tag2-aphT

Sm, Km

This study

S. Tm^{Δ4 TAG11}

Z2559

ΔsipAsopBsopEsopE2, Tag11-aphT

Sm, Km

This study

S. Tm^{Δ4 TAG13}

Z2560

ΔsipAsopBsopEsopE2, Tag13-aphT

Sm, Km

This study

S. Tm^{Δ4 TAG17}

Z2561

ΔsipAsopBsopEsopE2, Tag17-aphT

Sm, Km

This study

S. Tm^{Δ4 TAG19}

Z2562

ΔsipAsopBsopEsopE2, Tag19-aphT

Sm, Km

This study

S. Tm^{Δ4 TAG21}

Z2563

ΔsipAsopBsopEsopE2, Tag21-aphT

Sm, Km

This study

S. Tm^SPI-2-GFP

SB300

pssaG-GFPmut2

pssaG-GFPmut2 (pM975): expression of GFPmut2 under ssaG (SPI-2) promotor, which is active inside the SCV (S. Tm^SPI-2)

Sm, Amp

⁷²

S. Tm^SPI-1-GFP

Z1404

prgH-GFP

SPI-1 regulated expression of GFP (S. Tm^SPI-1)

Sm, Cm

¹⁰⁷

S. Tm^localizer

Z1440

ssaG-GFP

puhpT-mCherry

puhpT-mCherry (pCK100/pZ1400): Glc6P induced mCherry expression as a reporter for cytosolic localization (S. Tm^Glc6P). In addition, this strain contains an ssaG-driven GFP reporter for SCV localization (S. Tm^SPI-2)

Sm, Amp, Cm

This study

S. Tm^{Glc6p-mCherry}

Z1439

puhpT-mCherry

puhpT-mCherry (pCK100/pZ1400): Glc6P induced mCherry expression as a reporter for cytosolic localization (S. Tm^Glc6P).

Sm, Amp

This study

S. Tm^ΔfliGHI

M913

ΔfliGHI

pssaG-GFPmut2

Flagella-deficient mutant. Also contains pssaG-GFPmut2 (pM975): expression of GFPmut2 under ssaG (SPI-2) promotor, which is active inside the SCV (S. Tm^SPI-2)

Sm, Amp, Tet

¹⁰⁸

S. Tm^fliCind

Z2500

ΔflgB, Tag1-aphT

pEM087

Flagella-deficient mutant. Also contains pEM087: Doxycycline inducible expression of FliC

Sm, Cm, Amp, Tet, Km

¹⁴

S. Tm^ECV

Z2530

ΔflgB, Tag13-aphT

pZ2500

Flagella-deficient mutant. Also contains pZ2500: Empty control vector for pEM087 carrying a Tet (Doxycycline) resistance cassette

Sm, Cm, Amp, Tet, Km

¹⁴

14028

M3168

IpfED::aphT

Referred to as S. Tm¹⁴⁰²⁸

Km

⁹⁷

14028^TAG1

Z6708

IpfED::aphT, Tag1-cat

Collectively referred to as WITS¹⁴⁰²⁸

Km, Cm

This study

14028^TAG2

Z6709

IpfED::aphT, Tag2-cat

Km, Cm

This study

14028^TAG11

Z6710

IpfED::aphT, Tag11-cat

Km, Cm

This study

14028^TAG13

Z6711

IpfED::aphT, Tag13-cat

Km, Cm

This study

14028^TAG17

Z6712

IpfED::aphT, Tag17-cat

Km, Cm

This study

14028^TAG19

Z6713

IpfED::aphT, Tag19-cat

Km, Cm

This study

14028^TAG21

Z6714

IpfED::aphT, Tag21-cat

Km, Cm

This study