Fig. 3: Mucous cell hyperplasia in allergic asthmatic airways is associated with higher LASI expression in the airway epithelium. | Mucosal Immunology

Fig. 3: Mucous cell hyperplasia in allergic asthmatic airways is associated with higher LASI expression in the airway epithelium.

From: A long noncoding RNA antisense to ICAM-1 is involved in allergic asthma associated hyperreactive response of airway epithelial cells

Fig. 3

a Representative micrographs of airway sections of control and asthmatic subjects stained with Alcian blue for mucopolysaccharides and contrasted with H&E staining, scale – 5 μ. Quantification of AB + mucous cells (b) and eosinophils (c) per mm of basal lamina (BL). d Micrographs of airway sections stained for MUC5AC (green) and the DAPI-stained nuclei (blue) from control and asthmatic subjects, scale – 5 μ. e Quantification of MUC5AC + mucous cells per mm of BL in airways of controls and asthmatics. Relative quantities of MUC5AC (f), SPDEF (g), and LASI (h) in asthmatic HAECs compared to controls. i Micrographs of FISH-stained airway sections from control and asthmatic subjects showing LASI transcripts (red) and co-stained for MUC5AC (green) along with the DAPI-stained nuclei (blue), scale – 5 μ. Lower panels show the magnified image of the epithelial cells showing LASI transcripts in the inset drawn on the upper panels. j Quantification of LASI transcript per epithelial cell in airways of controls and asthmatics. Data shown as mean ± SEM; n = 6–7/gp; *p < 0.05; **p < 0.01; ****p < 0.0001 as analyzed by student’s t test.

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