Fig. 6: LPS treatment of ALI-cultured HAECs results in nuclear/perinuclear enrichment of LASI transcripts and increased expression of inflammatory factors. | Mucosal Immunology

Fig. 6: LPS treatment of ALI-cultured HAECs results in nuclear/perinuclear enrichment of LASI transcripts and increased expression of inflammatory factors.

From: A long noncoding RNA antisense to ICAM-1 is involved in allergic asthma associated hyperreactive response of airway epithelial cells

Fig. 6

a Micrographs of HAECs showing RNA FISH stained individual LASI transcripts (red) using RNAscope® technology and DAPI-stained nuclei (blue) in LPS-treated and non-treated (NT) HAECs, scale – 5 μ. b Quantification of the number of LASI transcripts per cell (denoted as H-score, see Methods for details) in LPS-treated and non-treated HAECs. Relative quantities of ICAM-1 (c), CXCL-8 (d), and IL-6 (e) mRNA levels in LPS-treated HAECs as determined by qRT-PCR at 24 h post-treatment in comparison with the non-treated controls. Data shown as mean ± SEM; n = 3–7/gp; *p < 0.05; ***p < 0.001 as analyzed by student’s t test.

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