Fig. 1: Batf3-dependent dendritic cells are required for the optimal induction of adaptive immunity toward Rotavirus.

a–c, f Batf3^WT/Het and Batf3^KO mice were orally infected with RV. a Levels of RV antigen shedding in feces was measured by ELISA. b The total number of total CD8⁺ T cells (left) and RV-specific CD8⁺ T cells (right) was assessed at 7 days post infection. c The levels of anti-RV IgA secreted into the feces after primary infection at indicated time-points was measured by ELISA. AUC = area under the curve, p value determined using an unpaired T test. d The levels of anti-RV IgA secreted into the feces before re-challenge at 8 weeks after primary infection (d0) and 4 days after rechallenge were measured by ELISA. e, f show kinetics for the total number of different B cells subsets accumulating in the mLNs after infection in C57Bl/6 mice (total B cells: live CD19⁺B220⁺, antigen-experienced: live CD19⁺B220⁺IgD⁻, plasmablasts: live CD19⁺IgD⁻CD138⁺) in BATF3 mice at 7 days post infection. Data pooled from a, b, d–f two (n = 4), or g six (n = 3–5) independent experiments, and c one representative experiment out of three (n = 4 mice each). a, c: unpaired T test, b, d–g: two-way ANOVA, Tukey post hoc. *p < 0.05, **p < 0.01, ***p < 0.001. White symbols: HET; black symbols: KO; shaded bars: RV-infected. AUC Area under the curve.