Fig. 2: BATF3-dependent cDC1 dendritic cells are required for the optimal induction of anti-RV-specific IgA responses in the mLNs.

a–c Batf3^Het and Batf3^KO mice were orally infected with RV. a Representative flow cytometry plots for RV-VLP staining in the B220⁺CD138⁻ B cells (pre-gated on CD19⁺IgD⁻) and the total number of IgA⁺ and IgM⁺ and RV-specific B cells in the mLNs. b Representative flow cytometry plots for RV-VLP staining in plasmablasts (B220⁻CD138⁺; pre-gated on CD19⁺IgD⁻) and the total number of IgA⁺ and IgM⁺ and RV-specific plasmablasts at 7 days post infection. c Representative flow cytometry plots for CCR9 and α₄β₇ expression on the different IgA⁺ plasmablasts (concatenated data from three mice, representative of two independent experiments). d huCD207-DTA and wildtype mice were orally infected with RV. The total number of IgA⁺ and RV-specific B cells (top) or plasmablasts at 7 days post infection. Cells were pre-gated as mentioned in a, b above. Dots represent data from individual mice. Data were collected from a, b six independent experiments, or d four independent experiments with 3–6 infected mice per experiment. Two-way ANOVA with Tukey post hoc test was performed for statistical analysis. *p < 0.05, **p < 0.01, ***p < 0.001. White symbols: HET; black symbols: KO; shaded bars: RV-infected.