Fig. 4: Blood neutrophilia of Gcnt1−/− mice is promoted by deficiency of this enzyme in hematopoietic cells, but increased susceptibility to Mtb infection also requires the non-hematopoietic compartment.
a Schematic representation of the BM transplantation model used and the experimental groups included. b The frequency of neutrophils in the blood of non-infected chimeric mice was determined by flow cytometry, following the gating strategy shown in Fig. S3. c Mice in the different chimeric groups were infected with a low dose of Mtb strain HN878 and the weight of the animals monitored to determine survival curves. d On day 30 post-infection, the bacterial burden in the lungs of the infected mice was determined by CFU enumeration. e H&E staining of one representative animal of each experimental group is represented. Black arrows point to edema, black arrowhead spot bronchopneumonia, and dashed black line limits the necrotic areas. Scale bar corresponds to 100 µm. f The number of neutrophils present in infected lungs was determined on day 30 post-infection, by flow cytometry. g The expression of Cxcl1, Cxcl2 and Cxcl5 in the lungs of infected mice was determined by real-time PCR, as above. b, d, f, g Represented is Mean±SEM, and each symbol represents one mouse. Statistical analysis was performed with a one-way ANOVA using Tukey’s test for multiple comparisons (b, d, f, g) or with log-rank (Mantel-Cox) test for the Kaplan Meyer curve (c). * refer to statistic differences between the indicated chimeric groups. **p < 0.01; ***p < 0.001; ****p < 0.0001.
