Fig. 5: rIL-18 supplementation ameliorates Prevotella-induced exacerbation of colonic inflammation. | Mucosal Immunology

Fig. 5: rIL-18 supplementation ameliorates Prevotella-induced exacerbation of colonic inflammation.

From: Perturbation of the gut microbiome by Prevotella spp. enhances host susceptibility to mucosal inflammation

Fig. 5

a Body weight loss of SPF and SPF + P mice during DSS colitis, either treated with PBS or 200 ng rIL-18. b Percent body weight and c histological evaluation of colon tissue on day 7 of DSS colitis of mice from (a), with d representative images of H&E-stained distal colon sections on d7 of DSS colitis. e Cytokines and f chemokines measured from distal colon tissue homogenates of SPF and SPF + P mice (d7 DSS) treated with PBS or rIL-18, analyzed using LEGENDplex kit or ELISA. g Relative abundance of P. intestinalis in in SPF + P mice before (untreated) and after rIL-18 treatment (rIL-18). Data represent n = 7–15 mice/group as mean ± SEM from one (d–g) or two representative experiments (a–c). P values are determined by two-way ANOVA by Tukey’s multiple comparison analysis (a–f) and paired nonparametric Wilcoxon test (g). *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001.

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