Fig. 5: Aging is marginally affecting the diversity of oral microbiota.
From: Niche rather than origin dysregulates mucosal Langerhans cells development in aged mice
a Total oral bacterial load was determined in oral swabs taken from young and aged mice by quantitative RT-PCR of the 16S rRNA gene. Bar graphs present the 16S/18S ratio in each group as the mean values + SEM (n = 8 per group). b CFU data of total cultivable oral anaerobic and aerobic bacteria sampled from young and aged mice. Representative data of one of two independent experiments with 8 mice/group + SEM. c–f Relative abundance of taxa in oral swabs sampled from young and aged mice. c α-diversity plot representing taxa richness in samples of both groups of mice (rarified to 6200 reads). d Principal coordinates analysis of weighted UniFrac distances based on 16S rRNA of both groups. e Pie charts representing the mean distribution of bacterial phyla among young and aged mice. f Histograms for individual mice represent the distribution of sequences in operational taxonomic units (OTUs) assigned to each family. g Relative abundance of high abundance bacterial families. Taxonomic data from one of two independent experiments are shown with 5–6 mice per group + SEM. h Absolute abundance of certain bacterial families in oral swabs collected from young and aged mice using quantitative RT-PCR analysis. Representative data of one of three independent experiments with 5 mice/group + SEM. *P < 0.05 (unpaired Student’s t test) compared with young samples.
