Fig. 7: Aged BM precursors efficiently differentiated to gingival LCs.

Representative FACS plots and graph show the gating strategies and frequencies of (a) pre-DCs and (b) common monocytes precursors (cMoP), monocytes and macrophage/DC precursors (MDP) in young and aged BM. Data are representative of three independent experiments with 5 mice/group + SEM. c Young and aged BM cells were cultured with GM-CSF and TGF-β1, FCAS plots and graph show the percentages of LC-like cells (MHCII⁺CD11c⁺EpCAM⁺DEC205⁺) in the cultures. Representative data of three independent experiments with 3–5 mice/group. d CD45.2⁺ aged mice and CD45.1⁺ young mice were lethally irradiated and then transplanted with a mixture (1:1) of BM cells from CD45.2⁺ aged and CD45.1⁺ young mice, the mice were analyzed 3 weeks after transplantation. e Representative FACS plots and graphs show the percentages of CD45.1⁺ vs. CD45.2⁺ B lymphocytes and monocytes in the blood of aged and young host. f FACS plots and graph demonstrate the frequencies of gingival LCs originating from young CD45.1⁺ vs. aged CD45.2⁺ BM precursors in the gingival epithelium of young and aged host. Data present the mean values of 5 mice/group + SEM. *P < 0.05, **P < 0.01 (unpaired Student’s t test) compared with young samples.