Fig. 1: Loss of CD47 reduces PMN TEpM in vivo. | Mucosal Immunology

Fig. 1: Loss of CD47 reduces PMN TEpM in vivo.

From: Neutrophil expressed CD47 regulates CD11b/CD18-dependent neutrophil transepithelial migration in the intestine in vivo

Fig. 1

a Schematic presentation of different fractions collected for neutrophil (PMN) quantification (left) and model of CD47 regulation of CD11b/CD18-dependent PMN transepithelial migration (TEpM) across intestinal mucosa (right). b Analysis of PMN TEpM in vivo using an ileal loop model reveals that Cd47−/− mice have significantly reduced numbers of PMN that migrated to the intestinal lumen 1 h after intraluminal instillation of leukotriene B4 (LTB4; 1 nM) compared with WT mice. Dots represent individual mice. Data are Means±SEM of three independent experiments, 14 mice/group. *p ≤ 0.05 as determined by Mann−Whitney U-test. c, d After luminal content collection, sections of intestinal loop were enzymatically digested to obtain a Lamina propria (LP)-enriched fraction or fixed and paraffin-embedded for histological analysis. c Quantification of leukocytes by flow cytometry show comparable numbers of PMN within the LP from WT and Cd47−/− mice. Data are Means±SEM of 2 independent experiments, 8 mice/group. d Representative images of immunohistochemical staining depicting Ly6G+ PMN. 20×(top) and 40×(bottom) objectives. Scale bars: 100 μm and 50 μm, respectively.

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