Fig. 2: Contributions of tissue-specific expression of CD47 to regulating PMN TEpM in vivo. | Mucosal Immunology

Fig. 2: Contributions of tissue-specific expression of CD47 to regulating PMN TEpM in vivo.

From: Neutrophil expressed CD47 regulates CD11b/CD18-dependent neutrophil transepithelial migration in the intestine in vivo

Fig. 2

a CD47 expression on bone marrow neutrophils (BMN) by western blotting and b densitometry analysis of three experiments reveal that nearly 80% of CD47 is selectively depleted on PMN from mice with specific deletion of CD47 on PMN (MRP8-Cre;Cd47fl/fl) compared to Cd47fl/fl control mice. c Reduced surface expression of CD47 was corroborated by flow cytometry on BMN from MRP8-Cre;Cd47fl/fl compared to Cd47fl/fl mice. d Analysis of PMN TEpM in vivo using a murine ileal loop model reveals significant reduction of transmigrated PMN into the intestinal lumen 1 h after intraluminal instillation of LTB4 (1 nM) in MRP8-Cre;Cd47fl/fl compared with Cd47fl/fl mice. Data are Means±SEM of at least three independent experiments, 16–18 mice/group. **p ≤ 0.01 as determined by Mann−Whitney U-test. e PMN TEpM in vivo in mice with specific deletion of CD47 on IECs (Villin-Cre;Cd47fl/fl) revealed similar numbers of PMN in the intestinal lumen 1 h after intraluminal instillation of LTB4 (1 nM) compared with Cd47fl/fl control littermates. Data are Means±SEM of 2 independent experiments, 6–9 mice/group.

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