Fig. 2: Ablation of Brg1 in ILC3s unleashes the proliferative capacity of ILC3s in a cell-intrinsic manner.
From: Brg1 restrains the pro-inflammatory properties of ILC3s and modulates intestinal immunity
a–c SI LPLs were isolated from neonatal or 14-day-old Rag1−/−Smarca4f/f or Rag1−/−Smarca4ΔILC3 mice. (n = 5–9; Representative of five experiments). a Histogram plots of flow cytometry analysis for RORγt frequency gated on Lin− cells. b Frequencies of ILC3s in Lin− cells. c Total numbers of ILC3s. d–g Mixed bone marrow chimeric mice were constructed as in Fig. S2 and sacrificed for analyses 6 weeks later. (n = 11–13; representative of six experiments) (d) FACS gating strategies of ILC3s in SI and LI LPLs of mixed bone marrow chimeric mice. e Frequencies of ILC3s from indicated hosts among all ILC3s identified by the congenic marker Thy1. f Representative flow cytometry plots showing gating strategy for analyzing Ki-67 expression in ILC3s from indicated hosts identified by the congenic marker Thy1 based on total Thy1+ILC3s gate. g Frequencies of Ki-67+ cells in ILC3s. a–g Data are represented as means ± SEM. Error bars show SEM. *P < 0.05; **P < 0.01; ***P < 0.001. Lin−, CD3ε−B220−CD11b−CD11c−.
