Fig. 4: Gpr84 was involved in the regulation of AMs and other primary macrophages. | Mucosal Immunology

Fig. 4: Gpr84 was involved in the regulation of AMs and other primary macrophages.

From: Regulatory role of Gpr84 in the switch of alveolar macrophages from CD11blo to CD11bhi status during lung injury process

Fig. 4

a Gpr84 expression in alveolar macrophage cell line MH-S under LPS challenge. b Expressions of ALI-related cytokines in MH-S with combined stimulations of LPS and GPR84 agonists; c Efficiency of Gpr84 knockdown in MH-S by RNA interference; d Expressions of ALI-related cytokines in MH-S after Gpr84 knockdown; e–g Expressions of ALI-related cytokines in Gpr84+/+ and Gpr84−/− AMs, BMDMs, and PEMs; h Phagocytosis of latex beads in Gpr84+/+ and Gpr84−/− AMs; i LPS-induced CD11b switch in Gpr84+/+ and Gpr84−/− AMs. Data of a–g are shown as means ± SEM (four biological repeats, three technical repeats), asterisk symbol indicates (*) compared with DMSO/NC/Gpr84+/+ cells under LPS challenge; data of h, i are shown as means ± SEM (four biological repeats, two technical repeats each), *0.01 < P < 0.05; **P < 0.01; ***P < 0.001.

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