Table 2 Primers used in real-time PCR.

From: Regulatory role of Gpr84 in the switch of alveolar macrophages from CD11b^lo to CD11b^hi status during lung injury process

Gene	F-/R-	Sequence (5′→3′)	PCR length (bp)
Arg1	Forward	CTCCAAGCCAAAGTCCTTAGAG	184
Arg1	Reverse	GGAGCTGTCATTAGGGACATCA	184
Ccl2	Forward	TAAAAACCTGGATCGGAACCAAA	120
Ccl2	Reverse	GCATTAGCTTCAGATTTACGGGT	120
Ccl3	Forward	TTCTCTGTACCATGACACTCTGC	100
Ccl3	Reverse	CGTGGAATCTTCCGGCTGTAG	100
Ccl4	Forward	TTCCTGCTGTTTCTCTTACACCT	121
Ccl4	Reverse	CTGTCTGCCTCTTTTGGTCAG	121
Ccl5	Forward	GCTGCTTTGCCTACCTCTCC	104
Ccl5	Reverse	TCGAGTGACAAACACGACTGC	104
Cd14	Forward	CTCTGTCCTTAAAGCGGCTTAC	191
Cd14	Reverse	GTTGCGGAGGTTCAAGATGTT	191
Cd206	Forward	CTCTGTTCAGCTATTGGACGC	132
Cd206	Reverse	CGGAATTTCTGGGATTCAGCTTC	132
Cd68	Forward	CCATCCTTCACGATGACACCT	132
Cd68	Reverse	GGCAGGGTTATGAGTGACAGTT	132
Cd86	Forward	GAGCTGGTAGTATTTTGGCAGG	100
Cd86	Reverse	GGCCCAGGTACTTGGCATT	100
Cxcl1	Forward	ACTGCACCCAAACCGAAGTC	114
Cxcl1	Reverse	TGGGGACACCTTTTAGCATCTT	114
Cxcl2	Forward	CCAACCACCAGGCTACAGG	108
Cxcl2	Reverse	GCGTCACACTCAAGCTCTG	108
Cxcl5	Forward	TCCAGCTCGCCATTCATGC	103
Cxcl5	Reverse	TTGCGGCTATGACTGAGGAAG	103
Fizz1	Forward	CCAATCCAGCTAACTATCCCTCC	188
Fizz1	Reverse	CCAGTCAACGAGTAAGCACAG	188
Nos2	Forward	GGAGTGACGGCAAACATGACT	127
Nos2	Reverse	TCGATGCACAACTGGGTGAAC	127
Gapdh	Forward	AGGTCGGTGTGAACGGATTTG	123
Gapdh	Reverse	TGTAGACCATGTAGTTGAGGTCA	123
Gpr40	Forward	GGGCATCAACATACCCGTGAA	192
Gpr40	Reverse	GCCCTGAGCTTCCGTTTGT	192
Gpr41	Forward	CTTCTTTCTTGGCAATTACTGGC	158
Gpr41	Reverse	CCGAAATGGTCAGGTTTAGCAA	158
Gpr43	Forward	CTTGATCCTCACGGCCTACAT	137
Gpr43	Reverse	CCAGGGTCAGATTAAGCAGGAG	137
Gpr84	Forward	CTCCTGCTACCATGAGTCTGT	186
Gpr84	Reverse	GTGCAGTAGAGTAGATCAGCCA	186
Gpr84 (identification)	Forward	AGGCCTGAGAATCTTTGTGAGCTA	172
Gpr84 (identification)	Reverse	GCCGGTACGCCAATGGAG	172
Gpr119	Forward	TCCTCACCGTCATGCTGATTG	159
Gpr119	Reverse	ATATGGAGACTCCGAGTGGGA	159
Gpr120	Forward	CTTCTGCGCGGATTTGCTC	123
Gpr120	Reverse	CCGCTCATTGTCATCACGTAGA	123
Il6	Forward	CTGCAAGAGACTTCCATCCAG	131
Il6	Reverse	AGTGGTATAGACAGGTCTGTTGG	131
Il12a	Forward	CTGTGCCTTGGTAGCATCTATG	169
Il12a	Reverse	GCAGAGTCTCGCCATTATGATTC	169
Il12b	Forward	TGGTTTGCCATCGTTTTGCTG	123
Il12b	Reverse	ACAGGTGAGGTTCACTGTTTCT	123
Il23a	Forward	AATAATGTGCCCCGTATCCAGT	142
Il23a	Reverse	GCTCCCCTTTGAAGATGTCAG	142
Il27	Forward	CTGTTGCTGCTACCCTTGCTT	177
Il27	Reverse	CACTCCTGGCAATCGAGATTC	177
Ebi3	Forward	CTTACAGGCTCGGTGTGGC	127
Ebi3	Reverse	GTGACATTTAGCATGTAGGGCA	127
Lbp	Forward	GATCACCGACAAGGGCCTG	143
Lbp	Reverse	GGCTATGAAACTCGTACTGCC	143
MD-2	Forward	CGCTGCTTTCTCCCATATTGA	140
MD-2	Reverse	CCTCAGTCTTATGCAGGGTTCA	140
Tlr4	Forward	TTTGACACCCTCCATAGACTTCA	114
Tlr4	Reverse	GAAACTGCAATCAAGAGTGCTG	114
Tnf	Forward	CCTGTAGCCCACGTCGTAG	148
Tnf	Reverse	GGGAGTAGACAAGGTACAACCC	148
Ym1	Forward	CAGGTCTGGCAATTCTTCTGAA	197
Ym1	Reverse	GTCTTGCTCATGTGTGTAAGTGA	197

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Table 2 Primers used in real-time PCR.

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