Fig. 3: Polyfunctional Mtb300-specific CD4 T cells (i.e CD153+ IL-2+ IFNγ+ TNFα+) are selectively reduced in aTB participants compared to LTBI, regardless of HIV status.

The x-axis displays each of the different response patterns, the composition of which is denoted with a dot for the presence of CD153, IL‐2, IFNγ, and TNFα. The proportion of each response pattern contributing to the total Mtb300-specific CD4 response per individual is shown. The median (gray bar) and interquartile ranges (box) are shown. Subsets accounting for <1% of the total Mtb300-specific CD4 T cell response are not displayed. Each response pattern is color‐coded, and data are summarized in the pie charts, where each pie slice represents the median contribution of each response pattern to the total Mtb300 response. A Wilcoxon rank-sum test was used to compare response patterns between groups (****P < 0.0001, ***P < 0.001, **P < 0.01). Statistical differences between pie charts were defined using a permutation test.