Fig. 2: Flagellin shifts the metabolic profile of HBE cells toward carbohydrates.
a Volcano plot representation (integrating multiple-test adjusted p values and log2-fold changes) of genome-wide transcriptome differences in HBE cells after incubation with flagellin or PBS (control) for 24 h. In red up-regulated genes; in blue downregulated genes. Horizontal line represents the multiple-test adjusted significance threshold (Benjamini–Hochberg adjusted p < 0.05). b Pathway analysis from a showing pathways enriched for up-regulated (red) or downregulated genes (blue). c Pathway analysis of transcriptional response in energy metabolism pathways in HBE cells upon flagellin stimulation for 24 h relative to unstimulated control cells. In red pathways enriched with up-regulated genes; in blue pathways enriched with downregulated genes. Adjusted p values were calculated using Benjamini–Hochberg adjustment. d Mitochondrial morphology assessed by electron microscopy. e Mitochondrial mass as measured by mitochondrial (mt)-DNA/genomic (g)-DNA ratio. p value was calculated using Student’s t test compared to unstimulated cells. ns not significant. f Reactive oxygen species (ROS) levels as measured with carboxy-H2DCFDA. p value was calculated using Student’s t test compared to PBS control. ns not significant. Data in a–c comprises three biological donors in duplicate from two independent experiments (n = 6). Data in e and f are displayed as box and whisker plots (n = 3–4 per group).
