Fig. 4: Akt-mTOR axis is triggered by flagellin and controls the glycolytic function of HBE cells. | Mucosal Immunology

Fig. 4: Akt-mTOR axis is triggered by flagellin and controls the glycolytic function of HBE cells.

From: mTOR-driven glycolysis governs induction of innate immune responses by bronchial epithelial cells exposed to the bacterial component flagellin

Fig. 4

a Immunoblot analysis of phosphorylated (p-) Akt and total Erk (loading control), in HBE cells from two donors, incubated with 1 μg/ml flagellin or PBS (control) for 2 h. b Immunoblot analysis of p-S6 and p-4EBP1, as well as total actin (loading control), in HBE cells from two donors (a, b) incubated with flagellin or PBS (control) for 2 h with or without preincubation (1 h) with 10 nM rapamycin. Levels of lactate secreted to the culture medium by HBE cells pretreated for 1 h with the mTOR inhibitors rapamycin (10 nM) (c), torin-1 (100 nM) (d), BEZ235 (100 nM) (e), or vehicle and stimulated for 24 h with PBS or flagellin. For c–e, data are presented as box and whisker plots (n = 4–7 replicates per group including two individual donors). p values were calculated using Student’s t test. *p < 0.05; **p < 0.01; ***p < 0.001; ns not significant.

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