Fig. 4: LTβR dampens neutrophil respiration and glycolysis. | Mucosal Immunology

Fig. 4: LTβR dampens neutrophil respiration and glycolysis.

From: Metabolic activation and colitis pathogenesis is prevented by lymphotoxin β receptor expression in neutrophils

Fig. 4

CD11b+ Ly6G+ Neutrophils were isolated from BM of Ltbrfl/fl × Mrp8-Cre (LTβRΔN) and control littermates (pooled from 5 mice/group) by magnetic sorting and their metabolic parameters were measured. a Oxygen consumption rate (OCR) and b extracellular acidification rate (ECAR) were measured in real time at baseline and in response to the indicated mitochondrial inhibitors. See “Methods” for details. c Basal and maximal mitochondrial respiration and glycolysis are mapped to illustrate pathway preference. ATP-linked respiration (d) and maximal respiratory capacity (e) are quantified as ΔOCR from flux analysis. Data from 5 mice/group, measured as mean of 5 wells for each timepoint, representing one of three independent experiments. Error bars represent SEM.

Back to article page