Fig. 5: Colon inflammation is associated with metabolic neutrophil activation in the absence of LTβR. | Mucosal Immunology

Fig. 5: Colon inflammation is associated with metabolic neutrophil activation in the absence of LTβR.

From: Metabolic activation and colitis pathogenesis is prevented by lymphotoxin β receptor expression in neutrophils

Fig. 5

Colitis was induced in Ltbr fl/fl × Mrp8-Cre (LTβRΔN) and control littermates by 2.5% DSS and disease and metabolic parameters were measured in colonic neutrophils. a Relative weight loss expressed as % of starting body weight. Quantification of neutrophil Mitotracker mean fluorescence intensity by flow cytometry (b) and representative histograms of colon neutrophil mitochondrial mass (c). d Mitochondrial superoxide production quantified by MitoSox by mean fluorescence intensity (left) and percent positive cells (right). Fluorescent glucose 2-NBDG uptake in neutrophils isolated from the colon quantified by flow cytometry (e) and representative histogram of 2-NBDG uptake (f). Student’s t test. Data from 3–4 mice/group, representative of 3 experiments. Error bars represent SEM.

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