Fig. 6: LTβR driven neutrophil metabolic activation causes exacerbated DSS-induced colitis.

Colitis was induced in Ltbr^fl/fl × Mrp8-Cre (LTβR^ΔN) and control littermates by 2.5% DSS. Metformin (Mtf, 200 mg/kg) or PBS was administered every other day by oral gavage and disease parameters were measured over time. a Weight loss was monitored daily as % starting body weight. b Colon length at endpoint of experiment. c H&E stained representative sections from distal colon with (d) blinded total histological score. Data represents three independent experiments (a, c) or is combined from three independent experiments (b, d). Significance tested by two-way ANOVA (a) or student’s t test (b, d). Scale bar, 200 μm. Error bars represent SEM.