Fig. 1: CD52 depletion ameliorates HDM-induced airway hyperreactivity (AHR) and abrogates inflammation.

a A group of WT mice (n = 6 mice) was intraperitoneally (i.p) immunized with 100 µg of house dust mite (HDM) in 2 mg of aluminum hydroxide (alum) and challenged with HDM (50 µg) or PBS intranasally (i.n.) on days 8, 9, and 10. Mice were treated with 250 µg of anti-CD52 depleting antibody (αCD52) i.p. or isotype control on day 7. We assessed the lung function and analyzed the BAL fluid on day 11, as shown in the timeline. b, c Line graphs show lung resistance and dynamic compliance (cDyn) in response to increasing doses of methacholine. Total numbers of CD45⁺ cells (d), number of eosinophils (e), number of T cells (f), and number of neutrophils (g) in the BAL fluid have been demonstrated in the bar graphs. h WT mice (n = 6 mice) were sensitized on day 1, and challenged intranasally with HDM on days 8, 9, and 10. Subsequently, the mice were treated with 250 µg of αCD52 or isotype control. The lung function and BAL were assessed on day 13. i Lung resistance. j Dynamic compliance. k Total numbers of CD45⁺ cells. l Number of eosinophils. m Number of T cells. n Number of neutrophils. o Representative images (left panel) and quantification (right panel) of hematoxylin and eosin-stained histologic sections of the lungs of mice. Scale bars = 50 μm. Data are shown as means ± SEMs and are representative of three individual experiments. Statistical analysis, two-tailed Student’s t-test. n.s. not significant, *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001. Mouse and lung images are provided with permission from Servier Medical Art.