Fig. 6: AHR agonists increase GPR15 expression in vivo.
From: The aryl hydrocarbon receptor regulates expression of mucosal trafficking receptor GPR15
Gpr15gfp/+ Ahr+/+ were orally gavaged with indole-3-carbinol or corn oil, or i.p. injected with TCDD and DMSO as shown in the figure. Representative FACS plots of gated CD4+ CD44hi CD45RBlo memory T cells of colon from vehicle- or agonist-treated mice. a Increased GPR15 (GFP+) memory CD4+ T cells in colon and small intestinal lamina propria with I3C. Mean + SEM, n = 11 corn oil, n = 11 I3C. Combined results of three experiments. b Increased GPR15 in colon, PLN, and spleen with 1 μg/kg doses of TCDD. n = 4 DMSO, n = 5 TCDD. c Increased GPR15 expression in intestinal and lymphoid tissues with 10 μg/kg doses of TCDD treatment. n = 9, except for spleen and PP, where n = 6. Combined results of three studies. For a–c, two-way ANOVA with Bonferroni posttest; *P < 0.05, ***P < 0.001, ****P < 0.0001; PLN peripheral lymph nodes, MLN mesenteric lymph nodes, PP Peyer’s patches, SI small intestine.
