Fig. 2: Contact-independent effect of coculture on eosinophil viability. | Mucosal Immunology

Fig. 2: Contact-independent effect of coculture on eosinophil viability.

From: Bidirectional crosstalk between eosinophils and esophageal epithelial cells regulates inflammatory and remodeling processes

Fig. 2

a Eosinophil survival and b CD69 MFI were assessed on eosinophils cultured for 96 h with media or media with IL-5 or cocultured in the bottom well in contact with EPC2s (“C”) or in the transwell insert with EPC2s in the bottom well (“T”). c Eosinophil viability was measured after culture with media alone, media with GM-CSF, or dilutions of EPC2-conditioned media (CM); results were anayzed by ANOVA with Dunnett’s post-test, and bars indicate p < 0.05. Data are representative of three biological replicates, each with 3–4 technical replicates per condition. a–c Data shown are mean ± SD and individual symbols represent technical replicates.

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