Fig. 4: Functional impact of soluble factors in coculture on eosinophil migration and viability. | Mucosal Immunology

Fig. 4: Functional impact of soluble factors in coculture on eosinophil migration and viability.

From: Bidirectional crosstalk between eosinophils and esophageal epithelial cells regulates inflammatory and remodeling processes

Fig. 4

a Eosinophils cultured for 24 h with media alone or media with IL-5 were seeded in porous inserts and allowed to migrate for 4 h in response to media, EPC2-conditioned media (CM), IL-13–treated EPC2-conditioned media or CCL26. b Viability was assessed after 14 days of culture with media alone or media with IL-5 or IL-13 or EPC2 coculture with or without IL-13. c Eosinophil viability was measured by Annexin-V staining and viability dye after 96 h of culture with either IL-5, GM-CSF, or EPC2-conditioned media (CM) with neutralizing antibody against GM-CSF or IgG control (Isotype). Data are representative of experiments that were repeated 2–3 times with three technical replicates per condition. Data shown are mean ± SEM, and individual symbols represent technical replicates. Significant differences were assessed with one-way ANOVA and Tukey post-test. Bars indicate p < 0.01, **p < 0.001. ND not detected.

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