Fig. 5: Tissue-specific responses of eosinophil gene expression to epithelial coculture. | Mucosal Immunology

Fig. 5: Tissue-specific responses of eosinophil gene expression to epithelial coculture.

From: Bidirectional crosstalk between eosinophils and esophageal epithelial cells regulates inflammatory and remodeling processes

Fig. 5

RNA extracted from freshly isolated eosinophils or nonadherent, CD45-enriched cells after 7 days of culture in media alone (baseline) or with IL-5 (IL-5) or cocultured with EPC2s (EPC2) or EPC2s with IL-13 (EPC2 IL-13) was sequenced. In the dataset, 6591 genes were expressed. a Of these, 3652 genes identified as significant by ANOVA (FDR < 0.05) and were clustered via K means (K = 6). Clusters were entered into GOrilla to obtain enrichments using total expressed genes as background. Selected processes and representative genes are annotated. b Representative individual genes are plotted. Data represent a single biological replicate with 2–4 technical replicates per condition. c Selected genes were quantified by RT-PCR using RNA from 2 to 5 technical replicates and three biological replicates. Significant differences were assessed with one-way ANOVA and Tukey post-test. Bars indicate p < 0.01. Data shown in b, c are mean ± SD and individual symbols represent technical replicates.

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