Fig. 1: miRNAs are differentially expressed in mice susceptible and resistant to H. polygyrus. | Mucosal Immunology

Fig. 1: miRNAs are differentially expressed in mice susceptible and resistant to H. polygyrus.

From: Inhibition of miR-99a-5p prevents allergen-driven airway exacerbations without compromising type-2 memory responses in the intestine following helminth infection

Fig. 1

a C57BL/6 mice were orally infected with 200 L3 H. polygyrus (H.p.) larvae on day 0. A cohort of mice was sacrificed 7 days-post 1° H. polygyrus infection (1°). Remaining mice were drug treated (Rx) on days 14 and 15. Two cohorts of mice were culled on days 42 and 63 after drug-cure (Rx early and Rx late, respectively). Two other cohorts of mice were then 2° challenge infected with H. polygyrus on day 35 or day 56 and harvested 7 days-post infection (2° early and 2° late, respectively). b The number of miRNAs significantly differentially expressed in the small intestine in 1°, Rx early, Rx late, 2° early and 2° late (up- and down-regulated, relative to naïve, p < 0.05). c Common and differentially expressed miRNAs in susceptible (1°) and resistant (2° early and 2° late) mice (relative to naïve, p < 0.05). d Expression profile of candidate miRNAs implicated in resistant to H. polygyrus identified in (c), n = 4. e miR-99a-5p, miR-148a-3p and miR-155-5p expression in small intestine from RNA sequencing data, n = 4, solid bars. Significance confirmed by qPCR, n = 8, grey circle. Data represented as mean ± SEM. *p < 0.05; **p < 0.01, determined using a one-way ANOVA with Dunnett’s multiple comparison analysis.

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