Fig. 7: Shotgun proteomics analysis of naive macrophage versus Eh-stimulated macrophage. | Mucosal Immunology

Fig. 7: Shotgun proteomics analysis of naive macrophage versus Eh-stimulated macrophage.

From: Entamoeba histolytica exploits the autophagy pathway in macrophages to trigger inflammation in disease pathogenesis

Fig. 7

a Shotgun proteomics protocol workflow. b Metascape analysis of different pathways between naive and Eh-stimulated macrophages. Blue marked are downregulated and red marked are upregulated pathways upon Eh interaction with macrophages. c Interesting protein hits identified within downregulated autophagy and vesicle-mediated transport pathways and with upregulated regulation of endopeptidase activity and antigen processing and presentation of peptide Ag pathways. d Bone marrow derived macrophages were stimulated with Eh for different time points and western blot done to detect ATG7 and SNAP23 levels in response to Eh and quantified by densitometric analysis. Cells without treatment is symbolized as (−ve). Eh-macrophage 1:20 ratio used. Data are representative of at least three independent experiments (n = 3) e STRING analysis of ATG16L1 protein-protein interaction with other known ATG proteins. **P < 0.01.

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