Fig. 2: Cigarette smoke exposure attenuates OVA-specific IgA ASC accumulation in the nasal mucosa after intranasal LPS/OVA immunization. | Mucosal Immunology

Fig. 2: Cigarette smoke exposure attenuates OVA-specific IgA ASC accumulation in the nasal mucosa after intranasal LPS/OVA immunization.

From: Cigarette smoke exposure attenuates the induction of antigen-specific IgA in the murine upper respiratory tract

Fig. 2

Mice exposed to room air (RA) or cigarette smoke (CS) for one week were intranasally immunized with LPS/OVA, and mice were analyzed at the indicated timepoints. a Nasal OVA-IgA ASC gating strategy (IgD–B220loCD138+IgG1–IgA+OVA+). b Flow cytometry plots demonstrating total ASCs (top), and OVA-IgA ASCs within IgA ASCs (bottom) in the nasal mucosa at 7dpb. Frequencies presented are mean ± SD. Absolute numbers of (c) total and (d) OVA-specific IgA ASCs were quantified in the nasal mucosa (NM) at the indicated timepoints. Data represent (c) n = 3–7 mice per group from two independent experiments, (d) n = 3–7 mice per group from three independent experiments. e Nasal OVA-IgA ASCs were quantified as a proportion of total IgA ASCs at 3dpb. Data represent n = 12 mice per group from two independent experiments. f Absolute number of OVA-IgA ASCs in the bone marrow quantified at 3dpb. Data represent n = 3–10 per group. FVD: fixable viability dye. e Student’s unpaired t-test, all others two-way ANOVA with Tukey’s post-hoc test. Mean ± SEM. *p < 0.05.

Back to article page