Fig. 4: The induction of antigen-specific IgA ASC responses is diminished in the NALT, CLNs and spleen in the context of cigarette smoke exposure. | Mucosal Immunology

Fig. 4: The induction of antigen-specific IgA ASC responses is diminished in the NALT, CLNs and spleen in the context of cigarette smoke exposure.

From: Cigarette smoke exposure attenuates the induction of antigen-specific IgA in the murine upper respiratory tract

Fig. 4

Mice exposed to room air (RA) or cigarette smoke (CS) for one week were intranasally immunized with LPS/OVA as described in Fig. 1, and NALTs, CLNs, spleen, and blood were assessed by flow cytometry at 3dpb. Total NALT ASCs are (A) visualized as flow cytometry plots within the parent gate (IgD– live singlets) and B quantified. C NALT OVA-IgA ASCs quantified as a percentage of total ASCs. NALT data points represent the means of pooled samples (n = 5–7 mice per group per experiment) from three independent experiments. D Pooled CLN cell count per mouse. E Absolute number of CLN OVA-IgA ASCs. F Spleen cell counts per mouse. Absolute number of OVA-IgA ASCs in the (G) spleen and (H) whole blood. Data represent: (D–G) n = 3–12 mice per group from two independent experiments, (H) n = 3–10 mice per group, one of two representative experiments is shown. Total NALT GCBCs are (I) visualized as flow cytometry plots within the parent gate (CD138–B220+) and (J) quantified. K NALT OVA-IgA GCBCs quantified as a percentage of total GCBCs. NALT data points represent the means of pooled samples (n = 5–7 mice per group per experiment) from two independent experiments. B, C, J, K Student’s unpaired t-tests, D–H two-way ANOVA with Tukey’s post-hoc test. Mean ± SEM. *p < 0.05.

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