Fig. 1: Tissue ILC2s have distinct signatures.
From: Tissue signals imprint Aiolos expression in ILC2s to modulate type 2 immunity
a, b, e, g, k, l Analysis of aggregated and downsampled single-cell data from tissue ILC2s was performed using Seurat. a t-SNE plot shows the distinct clusters of tissue ILC2s. b Heatmap shows the top 30 differentially expressed genes from tissue ILC2s performed using “1 versus all” comparison. e, g, k Violin plots show differentially expressed genes by tissue ILC2s. c, d, f, h, i, j Expression of ILC2 tissue-specific genes was verified in purified ILC2s from female mice (c, d, f, and j) or both genders (h and i) by real-time RT-PCR. c, d, f, h, i, j Heatmaps show relative fold of gene expression of representative tissue signatures relative to the expression by BM ILC2s. Black blocks indicate the gene was not detected in the tissue ILC2s by real-time RT-PCR. Scale bar indicates z-score of gene expression relative to Actb. l Gene ontology analysis was performed on LI and SI ILC2 common signature genes. BM bone marrow, LI large intestine, SI small intestine, P pancreas, L lung.
