Fig. 2: Tissue environment strongly shapes ILC2 identities.

a The expression of representative ILC2 tissue signatures gated on ILC2s was analyzed by flow cytometry. Intracellular 4-1BB was analyzed when cells were stimulated with PMA and ionomycin. Myeloid cell gate based on FSC and SSC was used as negative control. b Histogram shows Aiolos expression gated on human ILC2s from different tissues analyzed by flow cytometry. Negative control was Lin⁺ cells from bone marrow sample. c Schematic strategy of ILC2 transfer. d–g ILC2s were sorted from different tissues and transferred to Rag2^–/–Il2rg^–/– mice. Expression of Aiolos (d and e) and Gal-1 (f and g) gated on ILC2s (Lin^–GATA3⁺) from different tissues in Rag2^–/–Il2rg^–/– hosts was analyzed by flow cytometry 4 weeks after transfer. Mean fluorescence intensity (MFI) of Aiolos and Gal-1 is shown. h, i Mononuclear cells isolated from different tissues of the CD45.1 and CD45.2 mice were mixed at a 1:1 ratio and cultured for 48 h. The expression of Aiolos and Gal-1 gated on ILC2s (CD45.1⁺Lin^–GATA3⁺ cells) of CD45.1 origin was analyzed by flow cytometry. e–i Error bars are mean + SEM. a–i Data are representative of two to four independent experiments. BM bone marrow, LI large intestine, SI small intestine, P pancreas, L lung.