Fig. 5: ILC2 tissue-specific open chromatin regions (TS OCRs) correlate with ILC2 tissue signatures.
From: Tissue signals imprint Aiolos expression in ILC2s to modulate type 2 immunity
ATAC-seq analysis was performed using ILC2s isolated from different tissues. a PCA analysis based on normalized coverage of all combined peaks observed in tissue ILC2s. b Correlation analysis was performed on normalized coverage of ILC2 OCRs from indicated regions. Spearman correlation coefficients are shown. c Hierarchical clustering and heatmap was generated based on z-score of the normalized coverage of ILC2 TS OCRs. Representative ILC2 TS OCRs correlated ILC2 TS genes are shown. d Genome browser tracks with red boxes indicating representative ILC2 TS OCRs are shown. e Heatmap shows hierarchical clustering based on p value ILC2 TS OCRs-enriched motifs. White boxes indicate the motif is not found to be enriched in indicated ILC2 TS OCRs, or the p value was higher than the cutoff value (1 × 10−6). f mRNA expression of indicated genes from isolated tissue ILC2s was analyzed by real-time RT-PCR. Heatmap shows relative average expression of indicated genes normalized to the expression of BM ILC2s as 1. *indicates significant difference in increased expression of tissue-specific genes compared with each of the other tissue ILC2s analyzed from four biological samples.
