Fig. 3: Analysis of Cluster 35 indicates IEL. | Mucosal Immunology

Fig. 3: Analysis of Cluster 35 indicates IEL.

From: Human small intestinal infection by SARS-CoV-2 is characterized by a mucosal infiltration with activated CD8+ T cells

Fig. 3

a Imaging mass cytometry (IMC) image of CD8 staining (left) and cells designated to cluster 35 (right) overlaid with the computed cell masks. Scale bars represent 100 µm. b Single-cell tSNE maps show the expression of EpCAM, CD45, CD3, CD8, and Ki67. Plots show control (upper row) in comparison to COVID-19 (lower row) samples. The percentile cut-off was set to 99%. Color spectrum on the right of the plot indicates mean expression levels of the marker (red, high expression; blue, low expression). c Heatmap expression analysis of samples included in phenograph cluster 35 designated IEL. Marker expression levels columns are ordered according to hierarchical clustering. Dendrogram indicates similarity in marker expression. Color spectrum on the right of the plot indicates the mean expression levels of the marker (dark, high expression; light, low expression).

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