Fig. 3: STAT1 contributes to epithelial cell death during Citrobacter rodentium infection.
A-F Casp8ΔIEC, Casp8ΔIECxStat1−/− mice and control animals were orally infected with Citrobacter rodentium and analyzed at day 7 post infection. Pooled data of individual experiments (A, C n = 4; B, D–F n = 2). A Casp8ΔIEC (n = 13), Casp8ΔIECxStat1−/− (n = 13) mice and control mice (n = 16) were orally infected with Citrobacter rodentium. Relative body weight and Kaplan–Meier survival curve of infected animals. Error bars indicated +SD. B Representative images of H&E stained colon cross sections (scale bar: 100 μm) or immunohistochemically stained with an antibody against E-Cadherin (green) or stained with TUNEL assay (red). Nuclei were counterstained with Hoechst 33342 (blue) (scale bar: 50 μm). C Rel. bodyweight at day 7 post infection. D Histological score of H&E stained tissue cross sections. E Quantification of epithelial alteration of E-Cadherin staining visualized in B. F Gene transcription analysis of colonic mRNA expression. Hprt was used as housekeeping gene. Gene expression level is shown as fold change. Error bars indicate ±SD. Statistical analyses: One-way AOVA with Tukey’s multiple comparisons test; NS p ≥ 0.05; *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001.
